{
  "record_type": "published_pag_binding_summary_reanalysis",
  "schema": "dia-health-one.pag-binding-summary-reproduction.v1",
  "run": {
    "run_id": "DHO-PAG-BINDING-SUMMARY-20261018T111522Z",
    "created_at_utc": "2026-10-18T11:15:22.326502+00:00",
    "script": "research/reproduce_pag_binding_summary.py",
    "script_sha256": "473896f420e67620a4cdd31458d706e3502cffca4d28e15e15f761c772cdcb63",
    "input": "research/data/pag-binding-summary.csv",
    "input_sha256": "085800395b4eec8283064c5621fbe332e0760cfc6d1ebece65537b439da1c7ff"
  },
  "source": {
    "doi": "10.1021/acsptsci.6c00069",
    "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13270365/",
    "basis": "Published Kd estimates and rounded zero-glucose receptor-affinity means transcribed from the article."
  },
  "reported_values": [
    {
      "measure": "Kd",
      "condition": "D-glucose binding to NNC2215",
      "reported_value": "1.91",
      "reported_standard_error": "0.10",
      "uncertainty_status": "standard error",
      "unit": "mmol/L",
      "source_section": "Results: Binding of Glucose and PaG to NNC2215 In Vitro",
      "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13270365/"
    },
    {
      "measure": "Kd",
      "condition": "PaG binding to NNC2215",
      "reported_value": "0.17",
      "reported_standard_error": "0.01",
      "uncertainty_status": "standard error",
      "unit": "mmol/L",
      "source_section": "Results: Binding of Glucose and PaG to NNC2215 In Vitro",
      "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13270365/"
    },
    {
      "measure": "Relative insulin-receptor affinity",
      "condition": "zero d-glucose without PaG",
      "reported_value": "0.9",
      "reported_standard_error": "",
      "uncertainty_status": "rounded published mean; uncertainty not transcribed",
      "unit": "percent of human insulin",
      "source_section": "Results: Glucose- and PaG-Sensitive Binding of NNC2215 to the Insulin Receptor In Vitro",
      "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13270365/"
    },
    {
      "measure": "Relative insulin-receptor affinity",
      "condition": "zero d-glucose with 70 umol/L PaG",
      "reported_value": "5.4",
      "reported_standard_error": "",
      "uncertainty_status": "rounded published mean; uncertainty not transcribed",
      "unit": "percent of human insulin",
      "source_section": "Results: Glucose- and PaG-Sensitive Binding of NNC2215 to the Insulin Receptor In Vitro",
      "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13270365/"
    }
  ],
  "derived": {
    "pag_lower_kd_fold_vs_glucose": {
      "value": 11.235294117647058,
      "equation": "Kd_glucose / Kd_PaG",
      "unit": "fold",
      "interpretation": "Ratio of reported Kd means; a lower Kd indicates stronger binding."
    },
    "pag_receptor_affinity_fold_at_zero_glucose": {
      "value": 6.0,
      "equation": "relative_affinity_70uM_PaG / relative_affinity_no_PaG",
      "unit": "fold",
      "interpretation": "Ratio of rounded, published hIR-A relative-affinity means."
    },
    "pag_receptor_affinity_change_at_zero_glucose": {
      "value": 4.5,
      "equation": "relative_affinity_70uM_PaG - relative_affinity_no_PaG",
      "unit": "percentage points of human-insulin relative affinity"
    }
  },
  "limits": [
    "These calculations redivide published summary values; they do not fit the native-MS or receptor-binding data.",
    "The receptor-affinity inputs are rounded means. No uncertainty is calculated because the underlying replicate values and covariance are not transcribed here.",
    "This is an in-vitro summary reanalysis, not a molecular simulation or patient-specific prediction."
  ]
}